The Florey Advanced Microscopy Facility

State-of-the-art light microscopy systems and image processing tools plus ongoing support

Contact

Dr. Verena Wimmer



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Post author: Verena Wimmer. Last update: 29/10/2015 at 4:24 pm by Verena Wimmer.

Overview

The Florey’s Advanced Microscopy Facility (FAMF) offers a collection of state-of-the-art light microscopy equipment and image processing tools.

The FAMF supports in-house scientists and visitors in using light microscopy methods for their research. We also regularly organise in-house and international courses to teach basic and advanced light microscopy methods.

The FAMF presently manages 10 state of the art microscope systems from leading companies. Several workstations for image analysis are provided.

The FAMF provides access to:

  • 3 confocal microscopes (Zeiss LSM 780, Leica TCS SP8 and Olympus FV1000)
  • Inverted live cell imaging microscope (Olympus disc spinning unit; widefield fluorescence imaging)
  • 5 stereology workstations (brightfield and fluorescence, Stereoinvestigator and Neurolucida)
  • Dissecting microscope
  • MCID
  • 3 Image Analysis Workstations (Amira, Metamorph, Image-Pro Plus, ImageJ)
  • Server-based deconvolution (Huygens core)

The experienced microscopy team can assist users throughout the entire process of microscopic imaging: planning of an experiment, sample preparation, selection and usage of the microscope, image processing and visualisation, data transfer and storage.

All users of microscopy equipment need to be trained or demonstrate competence before access is permitted. Contact the platform to arrange training and platform induction.

Details

Benefits

We are passionate about quantitative imaging and the FAMF provides both state-of-the-art equipment and know how to quantify any aspect of your sample, from volume of structures of interest to number of neurons, synaptic contacts, neurite lengths and connectivity to co-localisation and dynamic processes in live cells. We provide server-based deconvolution using Huygens deconvolution software (SVI) to increase resolution and contrast, decrease noise and correct artefacts such as chromatic shifts, crosstalk and object movement.

Access
Instruments

Disc spinning unit (DSU) ARIN

Widefield and spinning disc live cell system used for live cell imaging.

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Huygens Core

Deconvolution server used for network accessible deconvolution.

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Imaging workstation 1 ARIN

Image analysis workstation used for 3D reconstruction and quantification.

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Imaging workstation 2 ARIN

Image analysis workstation used for manual and automated quantification.

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Imaging workstation 3 ARIN

Image analysis workstation used for Image processing.

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Leica DMLB ARIN

Upright fluorescence microscope used for stereology.

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Leica DMLB2 ARIN

Upright fluorescence microscope used for stereology, epi-fluorescence imaging.

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Leica TCS SP8 ARIN

Confocal laser scanning microscope used for optical sectioning, fixed tissue.

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MCID ARIN

Nikon Microphot SA with MCID analysis software used for densitometry.

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Nikon E800M Eclipse ARIN

Brightfield microscope with macro lenses used for large area imaging.

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Nikon Eclipse 80i ARIN

Bright field and fluorescence microscope used for neuron tracing.

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Olympus BX51 ARIN

Stereology/ Fluorescence microscope used for stereology and epi-fluorescence imaging.

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Olympus FV1000 ARIN

Confocal laser scanning microscope used for optical sectioning, live cell imaging.

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Zeiss LSM 780 ARIN

Confocal laser scanning microscope used for optical sectioning, fixed tissue.

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Services
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Location

The Florey Advanced Microscopy Facility

Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre (Parkville). Corner of Genetics Lane and Royal Parade, Parkville, ACT, Australia.

View location in Google maps.

Media

Categories


Type: Core facility in a research organisation, Research laboratory
Discipline areas: Biological Science
Precinct: Central
Affiliations: Neurosciences Victoria, The University of Melbourne
Accreditation: AQIS, OGTR
Biological Science categories: Computational, Confocal, Data storage, Digital, Fluorescence, High performance computing, Light, Live-cell, Microscopy, Quantitative image analysis, Widefield/deconvolution