State-of-the-art light microscopy systems and image processing tools plus ongoing support
Post author: Verena Wimmer. Last update: 29/10/2015 at 4:24 pm by Verena Wimmer.
The Florey’s Advanced Microscopy Facility (FAMF) offers a collection of state-of-the-art light microscopy equipment and image processing tools.
The FAMF supports in-house scientists and visitors in using light microscopy methods for their research. We also regularly organise in-house and international courses to teach basic and advanced light microscopy methods.
The FAMF presently manages 10 state of the art microscope systems from leading companies. Several workstations for image analysis are provided.
The FAMF provides access to:
- 3 confocal microscopes (Zeiss LSM 780, Leica TCS SP8 and Olympus FV1000)
- Inverted live cell imaging microscope (Olympus disc spinning unit; widefield fluorescence imaging)
- 5 stereology workstations (brightfield and fluorescence, Stereoinvestigator and Neurolucida)
- Dissecting microscope
- 3 Image Analysis Workstations (Amira, Metamorph, Image-Pro Plus, ImageJ)
- Server-based deconvolution (Huygens core)
The experienced microscopy team can assist users throughout the entire process of microscopic imaging: planning of an experiment, sample preparation, selection and usage of the microscope, image processing and visualisation, data transfer and storage.
All users of microscopy equipment need to be trained or demonstrate competence before access is permitted. Contact the platform to arrange training and platform induction.
We are passionate about quantitative imaging and the FAMF provides both state-of-the-art equipment and know how to quantify any aspect of your sample, from volume of structures of interest to number of neurons, synaptic contacts, neurite lengths and connectivity to co-localisation and dynamic processes in live cells. We provide server-based deconvolution using Huygens deconvolution software (SVI) to increase resolution and contrast, decrease noise and correct artefacts such as chromatic shifts, crosstalk and object movement.
The Florey Advanced Microscopy Facility
Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre (Parkville). Corner of Genetics Lane and Royal Parade, Parkville, ACT, Australia.
View location in Google maps.
Type: Core facility in a research organisation, Research laboratory
Discipline areas: Biological Science
Affiliations: Neurosciences Victoria, The University of Melbourne
Accreditation: AQIS, OGTR
Biological Science categories: Computational, Confocal, Data storage, Digital, Fluorescence, High performance computing, Light, Live-cell, Microscopy, Quantitative image analysis, Widefield/deconvolution