Peter MacCallum Cancer Centre
Post author: Sarah Ellis. Last update: 30/10/2015 at 11:39 am by Sarah Ellis.
The Microscopy Core is composed of four overlapping areas; histology, optical microscopy, electron microscopy (EM) and image analysis.
- Histology: All aspects of histology including wax, resin, frozen sectioning and immunohistochemistry for research.
- Optical microscopy: Immunofluorescence, live cell microscopy (wide-field,confocal and spinning disk), laser capture microscopy,and confocal microscopy.
- Electron microscopy (both scanning and transmission): Cryo-ultramicrotomy, freeze fixation (both slamming/plunging and high pressure fixation), PLT (progressive lowering of temperature), freeze substitution, chemical fixation,immunogold labelling, correlative optical/electron microscopy, critical point drying including a fully automated system, gold and carbon sputter coating and all ancilliary equipment for specimen preparation.
- Image analysis: Image analysis of micrographs including live cell tracking, quantitation of immunohistochemistry/fluorescence, cell shape/size/volume, and cell counting.
The experienced microscopy staff provide full support for researchers utilising the facility’s microscopes and are available for Consultation/collaboration/training. Regular mini-workshops are also carried out.
- Research collaboration.
- Fee for service.
- Expertise in immuno-electron microscopy.
- Expertise in live cell microscopy.
- Extensive experience in histology for researchers (light sensitive work, serials etc).
Expertise:Availability of technical support:
- Support for experimental design is available.
- Support for instrument use is available.
Note that this material was imported form the Victorian Bioportal and content requires updating.