Peter MacCallum Cancer Centre Microscopy Core Facility

Peter MacCallum Cancer Centre

Contact

Dr. Sarah Ellis

(613) 9656 1244


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Post author: Sarah Ellis. Last update: 30/10/2015 at 11:39 am by Sarah Ellis.

Overview

The Microscopy Core is composed of four overlapping areas; histology, optical microscopy, electron microscopy (EM) and image analysis.

  • Histology: All aspects of histology including wax, resin, frozen sectioning and immunohistochemistry for research.
  • Optical microscopy: Immunofluorescence, live cell microscopy (wide-field,confocal and spinning disk), laser capture microscopy,and confocal microscopy.
  • Electron microscopy (both scanning and transmission): Cryo-ultramicrotomy, freeze fixation (both slamming/plunging and high pressure fixation), PLT (progressive lowering of temperature), freeze substitution, chemical fixation,immunogold labelling, correlative optical/electron microscopy, critical point drying including a fully automated system, gold and carbon sputter coating and all ancilliary equipment for specimen preparation.
  • Image analysis: Image analysis of micrographs including live cell tracking, quantitation of immunohistochemistry/fluorescence, cell shape/size/volume, and cell counting.

The experienced microscopy staff provide full support for researchers utilising the facility’s microscopes and are available for Consultation/collaboration/training. Regular mini-workshops are also carried out.

Other infrastructure:

    Access by:

    • Research collaboration.
    • Fee for service.

Details

Benefits

Know how:

  • Expertise in immuno-electron microscopy.
  • Expertise in live cell microscopy.
  • Extensive experience in histology for researchers (light sensitive work, serials etc).

Expertise:Availability of technical support:

  • Support for experimental design is available.
  • Support for instrument use is available.

Note that this material was imported form the Victorian Bioportal and content requires updating.

Access
Instruments

AFS II

Automatic Freeze Substitution apparatus used for Substitution of water with solvent/resin at very low temperatures.

Facility: |

JEOL Neoscope II

Scanning Electron Microscope used for Scanning Electron Microscopy.

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Leica EM CPD300

Critical Point Dryer used for Critical Point Drying.

Facility: |

Ultracut FC7 with UC7 cryoattachment

Ultramicrotome with cryo used for Cutting very thin sections for TEM.

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Services
No services found

Location

Peter MacCallum Cancer Centre Microscopy Core Facility

Peter MacCallum Cancer Centre, Research Division. St Andrews Place, East Melbourne, Victoria, Australia.

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Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: Central
Affiliations: Peter MacCallum Cancer Centre, VMN

Biological Science categories: Cells and tissues, Confocal, Electron, Fluorescence, Histology, In-vivo imaging, Light, Live-cell, Microscopy, Mulit-modal, Optical, Quantitative image analysis, Widefield/deconvolution