Mouse Metabolic Phenotyping Facility

Baker IDI Heart & Diabetes Institute

Contact

Mr. Mark Febbraio

(613) 8532 1111


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Post author: Mark Febbraio. Last update: 30/10/2015 at 3:17 pm by Mark Febbraio.

Overview

Mice can be shipped to our Facility to obtain a range of complex exams (on a fee-for-service basis) used to characterize mouse metabolism, blood composition including hormones, energy balance and physical activity, eating and exercise, insulin resistance, metabolic fluxes and physiology. Many tests are done in living animals and are designed to elucidate subtle to complex traits that would define models of metabolic disease.

Details

Benefits

Know how:The major services offered are:

  • Body composition analysis: Body fat, lean mass, fluids and total water is measured in liver mice and rats weighing 10-700 grams.
  • Indirect calorimetry / Energy expenditure: Whole body oxygen consumption and carbon dioxide production is measure continuously in conscious mice and rats using a Comprehensive Laboratory Animal Monitoring System. The system can be used to measure resting or exercising gas exchange and energy expenditure.
  • Assessment of glucose and insulin tolerance test: Glucose and insulin tolerance tests are conducted in conscious mice and rats.
  • Skeletal muscle fatty acid metabolism: Skeletal muscle fatty acid metabolism is assessed in isolated muscle of mice and rats. Tracer methodology is use to determine rates of fatty acid oxidation, incorporation into endogenous lipid pools and lipolysis.
  • Jugular vein catheterization: A catheter is chronically implanted into the jugular vein of mice and rats. Catheterization of the jugular vein allows the infusion of hormones, substrates, and tracers into the systemic circulation.
  • Euglycemic hyperinsulinemic clamp: The euglycemic hyperinsulinemic clamp is used to measure insulin action in vivo. Euglycemic hyperinsulinemic clamps are performed on conscious mice and rats with catheters chronically implanted in the jugular vein. A continuous infusion of insulin is given. Glucose levels are monitored every 5-10 min. Glucose is infused in the jugular vein catheter at rates necessary to achieve the desired glucose level, based on feedback from glucose measurements. These methods allow assessment of the responsiveness of the body to insulin. By combining this technique with the tracer method one can also examine the impact of insulin on suppression of endogenous glucose production as well as measuring tissue specific glucose uptake.

Expertise:Availability of technical support:

  • Technical support is available

Note that this material was imported form the Victorian Bioportal and content requires updating.

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Instruments
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Location

Mouse Metabolic Phenotyping Facility

Baker IDI Heart and Diabetes Institute. 75 Commercial Rd, Prahran, Victoria, Australia.

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Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: Central
Affiliations: Baker IDI Heart & Diabetes Institute

Biological Science categories: Animal models, Bioresources, Metabolomics, Mouse