Peter MacCallum Cancer Centre, Research Division.
Post author: Gisela Mir Arnau. Last update: 02/11/2015 at 12:05 pm by Gisela Mir Arnau.
The Molecular Genomics facility at Peter Mac operates two major platforms; Illumina HiSeq 2500 (next-generation sequencer) and Nanostring nCounter Analysis System (digital analyser).
The llumina HiSeq 2500 is used worldwide as the current industry standard in next-generation sequencing. Key applications in our facility include DNA resequencing for mutation discovery (exome sequencing and custom targeted capture), transcriptome sequencing (RNA-seq) and epigenomics applications such as ChIP-seq.
The NanoString nCounter Analysis System represents a new wave of genomic technologies that enables the rapid and reliable analysis of nucleic acids at single-molecule resolution. The platform has a broad range of applications including transcription (mRNA, miRNA and lncRNA), copy-number number analysis, detection of cancer-translocations and ChIP-enriched DNA (ChIP-string).
The core also houses ancillary equipment for sample preparation and quality control including an epMotion robotic liquid handling system (Eppendorf), Bioanalyzer 2100 and TapeStation (Agilent), Synergy HT Microplate Reader (BioTek), S2 Ultrasonicator (Covaris) or Qubit fluorometer (Life Technologies).
We have experience with small amounts of input material and the use of DNA/RNA from archival formalin-fixed paraffin embedded (FFPE) samples.
Research Division’s Bioinformatics supports our Next Gen Seq and Nanostring data analysis.
In collaboration with the Victorian Centre for Functional Genomics (VCFG) we have developed readout by Next Gen Seq of pooled shRNA gene knockdown screens.