Melbourne Mass Spectrometry and Proteomics.

The University of Melbourne, Bio21 Institute.

Contact

Dr. Nicholas Williamson

(613) 8344 2206


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Post author: Nicholas Williamson. Last update: 30/10/2015 at 2:29 pm by Nicholas Williamson.

Overview

Do it yourself Mass Spec and HPLC.

This facility has seven mass spectrometers and associated HPLC systems plus all the bioinformatics infrastructure required for proteomic research. While fee for service work is available, for a fixed (highly subsidized) yearly or quarterly fee we also offer unlimited* instrument access and training to users who wish to perform their own mass spectrometry experiments. The facility has four full time staff, each with over 15 years of protein mass spectrometry experience to assist users to extract maximum benefit from the instrumentation available. Primary structure analysis, disulfide arrangements, phosphorylation site analysis, iTRAQ, SILAC etc are routinely performed. Instrumentation includes two Agilent LC-6220 esi-TOF, Sciex 5500QTrap, Thermo OrbiTRAP ELITE ETD, and Thermo QExactive Plus. Four Agilent HPLC systems are also available for purification of samples. Bookings can be made here once users are registered: http://apps.bio21.unimelb.edu.au/booking/select_resource.php?v=1

Other infrastructure:

  • Four Agilent HPLC systems.
  • Freeze Dryer
  • Speed Vac

Access by:

  • Research collaboration.
  • Membership

Details

Benefits

Know how:

  • Proteomics.
  • Protein primary structure analysis.
  • Posttranslational modifications.
  • Recombinant protein QC
  • synthetic peptide QC
  • iTRAQ, SILAC, Label free proteomic methods
  • Post-translational modifications

Expertise:Availability of technical support:

  • Support for experimental design is available.
  • Support for instrument use is available.
  • Grant writing assistance is available
Access

Our costs are based on a membership structure.  The facility access cost is highly subsidized by the University of Melbourne and is charged on a quarterly, or yearly basis.  A laboratory head will pay the subscription cost ($4400 for University of Melbourne, $5900 for external academic) and then every laboratory member can access the platform.

We have more than 300 users from more than 70 different labs all over Victoria and Interstate as evidence that this approach is very effective.  We have a fleet of eight mass spectrometers and we make it as easy as possible for you to learn as much or as little as you like about the actual instrumentation.

Access by fee for service
Access by users external to managing organisation, Access by users internal to managing organisation

Instruments

Agilent 6220 esiTOF

Time of Flight Mass spectrometer used for mass spectrometry.

Facility: Dr. Nicholas Williamson | (613) 8344 2206

OrbiTRAP ELITE ETD

Mass spectrometer used for proteomics.

Facility: Dr. Nicholas Williamson | (613) 8344 2206

QExactive Plus

Mass spectrometer used for proteomics.

Facility: Dr. Nicholas Williamson | (613) 8344 2206

Services

HPLC Purification

Used for Purification of chemical synthesis or protein mixtures.

Facility contact: Dr. Nicholas Williamson | (613) 8344 2206

Proteomic mass spectrometry

Used for Proteomic Mass Spectrometry.

Facility contact: Dr. Nicholas Williamson | (613) 8344 2206

Location

Melbourne Mass Spectrometry and Proteomics.

The University of Melbourne, Bio21 Institute. 30 Flemington Road, Parkville, Victoria, Australia.

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Media

Categories


Type: Commercial research services provider, Core facility in a research organisation, Research center, Research laboratory
Discipline areas: Biological Science, Chemical Science, Engineering Science, Food Science
Precinct: Central
Affiliations: PMV, The University of Melbourne

Biological Science categories: ADME, Amino acid and sequencing analysis, Analytical assays, Bioinformatics, Clinical trials resources, Computational, Computational Biology, Drug development, HPLC, HPLC, LC, Mass spectroscopy, Mass spectroscopy, Mass spectroscopy, Metabolomics, Peptide synthesis, Post-translational modification, Properties and interactions, Protein Identification, Protein Purification, Proteins and peptides, Proteomics, Quantitation, Recombinant proteins

Tags: mass spectrometry, Protein, proteomics