The University of Melbourne, Bio21 Institute.
Post author: Nicholas Williamson. Last update: 30/10/2015 at 2:29 pm by Nicholas Williamson.
Do it yourself Mass Spec and HPLC.
This facility has seven mass spectrometers and associated HPLC systems plus all the bioinformatics infrastructure required for proteomic research. While fee for service work is available, for a fixed (highly subsidized) yearly or quarterly fee we also offer unlimited* instrument access and training to users who wish to perform their own mass spectrometry experiments. The facility has four full time staff, each with over 15 years of protein mass spectrometry experience to assist users to extract maximum benefit from the instrumentation available. Primary structure analysis, disulfide arrangements, phosphorylation site analysis, iTRAQ, SILAC etc are routinely performed. Instrumentation includes two Agilent LC-6220 esi-TOF, Sciex 5500QTrap, Thermo OrbiTRAP ELITE ETD, and Thermo QExactive Plus. Four Agilent HPLC systems are also available for purification of samples. Bookings can be made here once users are registered: http://apps.bio21.unimelb.edu.au/booking/select_resource.php?v=1
- Four Agilent HPLC systems.
- Freeze Dryer
- Speed Vac
- Research collaboration.
- Protein primary structure analysis.
- Posttranslational modifications.
- Recombinant protein QC
- synthetic peptide QC
- iTRAQ, SILAC, Label free proteomic methods
- Post-translational modifications
Expertise:Availability of technical support:
- Support for experimental design is available.
- Support for instrument use is available.
- Grant writing assistance is available
Our costs are based on a membership structure. The facility access cost is highly subsidized by the University of Melbourne and is charged on a quarterly, or yearly basis. A laboratory head will pay the subscription cost ($4400 for University of Melbourne, $5900 for external academic) and then every laboratory member can access the platform.
We have more than 300 users from more than 70 different labs all over Victoria and Interstate as evidence that this approach is very effective. We have a fleet of eight mass spectrometers and we make it as easy as possible for you to learn as much or as little as you like about the actual instrumentation.
Agilent 6220 esiTOF
Time of Flight Mass spectrometer used for mass spectrometry.
Facility: Dr. Nicholas Williamson | (613) 8344 2206
OrbiTRAP ELITE ETD
Type: Commercial research services provider, Core facility in a research organisation, Research center, Research laboratory
Discipline areas: Biological Science, Chemical Science, Engineering Science, Food Science
Affiliations: PMV, The University of Melbourne
ADME, Amino acid and sequencing analysis, Analytical assays, Bioinformatics, Clinical trials resources, Computational, Computational Biology, Drug development, HPLC, HPLC, LC, Mass spectroscopy, Mass spectroscopy, Mass spectroscopy, Metabolomics, Peptide synthesis, Post-translational modification, Properties and interactions, Protein Identification, Protein Purification, Proteins and peptides, Proteomics, Quantitation, Recombinant proteinsBiological Science categories: