Melbourne Advanced Fluorescence Imaging Platform

Cutting-edge optical microscopy and flow cytometry

Contact

Mr. Paul Brannon

90357749


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Post author: Lauren Hyde. Last update: 02/11/2015 at 10:39 am by Lauren Hyde.

Overview

The Advanced Fluorescence Imaging Platform provides access to cutting-edge optical microscopy and flow cytometry for researchers at the University of Melbourne, as well as the wider community. The platform focuses on providing access to three key techniques; super-resolution microscopy, live cell imaging and imaging flow cytometry. These three complementary techniques benefit researchers in the fields of nanomedicine, immunology, materials engineering, cell biology and cancer research and spans the faculties of Engineering, Science and Medicine. Furthermore, the multi-disciplinary nature of the platform will foster collaboration between microscopists, cell biologists, physicists and material scientists.

Until recently, the highest resolution that can be achieved using optical microscopy has been limited to ~250nm. However, many nanoengineered materials and structures within cells, such as nanocapsules and cellular membranes are smaller than this limit. The super-resolution microscopes at the AFI platform offer the ability to break this resolution limit and resolve structures down to 20 nm. However, while super-resolution microscopy at the nanometer scale gives unprecedented insight into cellular systems, it is also important to understand interactions in living cells. The AFI platform offers super-resolution live-cell imaging down to 100 nm, as well as conventional live-cell imaging.

Imaging flow cytometry bridges the gap between microscopy and flow cytometry. While microscope based systems have low throughput, flow cytometry is unable to acquire structural information. Imaging flow cytometry enables structural information to be gathered at a rate in excess of 100 cells per second, allowing meaningful statistical information to be obtained. The AFI is the only facility in Australia to combine Super-Resolution Microscopy, Live Cell imaging and Imaging Flow Cytometry.

These instruments take significant skill and expertise to operate. To facilitate access, a full-time platform support officer is available to run samples and train repeat users. Due to the specialised nature of operation, the super-resolution microscopes (Nikon N-STORM and OMX BLAZE) are only available for assisted use with the platform support officer.

Details

Benefits

The Advanced Fluorescence Imaging platform has particular expertise in;

  • super-resolution techniques such as SIM and STORM/PALM
  • fluorescence imaging and analysis from TIRF, confocal, deconvolution and flow cytometry
  • experiment design for complementary super-resolution microscopy, flow cytometry and live-cell imaging
  • developing molecular sensors for biological sensing
  • studying the in vitro behaviour of nanomaterials
  • applying super-resolution imaging techniques for material characterisation
Access

To access the instrumentation, either as a collaboration or fee for service, please contact the platform support officer Paul Brannon. We welcome new users and collaborators, and can provide guidance for experimental design and reagent selection. We also stock a range of fluorescent antibodies, organelle stains and sensors to help users optimise their staining protocols. Existing users can make instrument bookings here.

Access by collaboration, Access by fee for service
Access by users external to managing organisation, Access by users internal to managing organisation

People

Mr. Paul Brannon

Mr. Paul Brannon

Facility Staff at The University of Melbourne, Chemical and Biomolecular Engineering.

P: 90357749 | Email |

Instruments

Amnis Image Stream

Imaging Flow Cytometry used for imaging flow cytometry.

Facility: |

Apogee A-50 Microflow

Flow Cytometer used for high-sensitivity flow cytometry.

Facility: |

Deltavision Deconvolution

Deconvolution Microscope used for live cell imaging, fluorescence microscopy.

Facility: |

Leica TCS SP2 Confocal

Confocal Microscope used for 3D imaging, confocal imaging, live cell imaging, FLIM.

Facility: |

Nikon N-STORM

Super-resolution microscope used for STORM, PALM, TIRF imaging, fluorescence microscopy.

Facility: |

OMX V4 Blaze

SIM super-resolution microscope used for structured illumination microscopy, live cell imaging, high speed deconvolution microscopy.

Facility: |

Partec Cyflow Space

Flow cytometer used for flow cytometry.

Facility: |

Location

Melbourne Advanced Fluorescence Imaging Platform

The University of Melbourne, . Grattan Street, Parkville, Victoria, Australia.

View location in Google maps.

Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science, Chemical Science, Engineering Science, Materials Science, Nano Science
Precinct: South east
Affiliations: The University of Melbourne

Biological Science categories: AFM, Confocal, Count, Digital, Flow cytometry, Fluorescence, Light, Live-cell, Microscopy, Super resolution, Two-photon, Widefield/deconvolution

Tags: confocal, deconvolution microscopy, FLIM, Flow cytometry, fluorescence microscopy, imaging flow cytometry, super-resolution