A one-stop service for high quality recombinant proteins and monoclonal antibodies
Post author: MPEF. Last update: 30/10/2015 at 2:51 pm by MPEF.
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The MPEF is a unique facility that can produce small to large amounts of high quality recombinant proteins and antibodies for use in medical research.
Cell Line Development
Transient expression, stable expression, and isolation / enrichment of high producing clones can be performed.
A range of production systems are available depending on the scale of production required. Perfusion systems are able to produce material every two days ranging from tens of milligrams to several hundreds of milligrams per harvest. A proprietary biphasic approach to production is used during protein production in stirred tank bioreactors. Yields up to 2g/L of mAb in a GS CHO cell line can be achieved.
Purification and Product Characterisation
Production material is purified using a variety of chromatography techniques such as affinity, size exclusion and ion exchange. Operating procedures are in place to allow for flexibility to process materials ranging from milligrams to several grams of material.
Quality assessments are conducted through a combination of SDS-PAGE, size exclusion chromatography using HPLC, and binding kinetics using BIAcore.
- Cell line development
- Biologics production
- Protein purification
- Protein analysis
- Protein characterisation
Dr. Angelo Perani
Mr. Mark Frewin
Dr. Bruno Catimel
Dr. Benjamin Gloria
Mammalian Protein Expression, Production and Purification Facility (MPEF)
Olivia Newton-John Cancer Research Institute. 145 Studley Road, Heidelberg, Victoria, Australia.
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