Mammalian Protein Expression, Production and Purification Facility (MPEF)

A one-stop service for high quality recombinant proteins and monoclonal antibodies

Contact

Dr. Angelo Perani

61-3-94965366


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Post author: MPEF. Last update: 30/10/2015 at 2:51 pm by MPEF.

Special offer

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Overview

The MPEF is a unique facility that can produce small to large amounts of high quality recombinant proteins and antibodies for use in medical research.

Cell Line Development
Transient expression, stable expression, and isolation / enrichment of high producing clones can be performed.

Production Systems
A range of production systems are available depending on the scale of production required. Perfusion systems are able to produce material every two days ranging from tens of milligrams to several hundreds of milligrams per harvest. A proprietary biphasic approach to production is used during protein production in stirred tank bioreactors. Yields up to 2g/L of mAb in a GS CHO cell line can be achieved.

Purification and Product Characterisation
Production material is purified using a variety of chromatography techniques such as affinity, size exclusion and ion exchange. Operating procedures are in place to allow for flexibility to process materials ranging from milligrams to several grams of material.
Quality assessments are conducted through a combination of SDS-PAGE, size exclusion chromatography using HPLC, and binding kinetics using BIAcore.

Details

Benefits

Specialising in:

  • Cell line development
  • Biologics production
  • Protein purification
  • Protein analysis
  • Protein characterisation
Access

Access is available to anyone requiring recombinant proteins produced. Please contact the Facility Manager

Access by fee for service
Access by users external to managing organisation, Access by users internal to managing organisation

People

Dr. Angelo Perani

Dr. Angelo Perani

Facility Manager at Olivia Newton-John Cancer Research Institute, .

P: 61-3-94965366 | Email |

Mr. Mark Frewin

Mr. Mark Frewin

Laboratory Manager at Olivia Newton-John Cancer Research Institute, .

Email |

Dr. Bruno Catimel

Dr. Bruno Catimel

Facility Staff at Olivia Newton-John Cancer Research Institute, .

Email |

Dr. Benjamin Gloria

Dr. Benjamin Gloria

Facility Staff at Olivia Newton-John Cancer Research Institute, .

Email |

Instruments
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Services
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Location

Mammalian Protein Expression, Production and Purification Facility (MPEF)

Olivia Newton-John Cancer Research Institute. 145 Studley Road, Heidelberg, Victoria, Australia.

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Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: Northern
Affiliations: Olivia Newton-John Cancer Research Institute

Biological Science categories: Cell culture, Cells and tissues, Protein Purification, Proteins and peptides, Recombinant proteins