LIMS Microscopy and Imaging Facility

Latrobe Institute for Molecular Science

Contact

Dr. Peter Lock

61 3 9479 3349


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Post author: Peter Lock. Last update: 30/10/2015 at 1:36 pm by Peter Lock.

Overview

The LIMS Microscopy and Imaging Facility offers academic and industry users access to $2m of  sophisticated light microscopy equipment for biological imaging projects. We train and support researchers to independently operate instruments on a fee for use basis.

High-speed live cell fluorescence imaging is a core capability of our facility enabling users to faithfully record dynamic biological processes in cells, especially molecular interactions, with full incubation control. A fast and sensitive spinning disk confocal system with dual EMCCD cameras is available for synchronous 2-channel imaging; this system is designed for rapid Z-stack acquisition during time lapse experiments, enabling users to record 3D movies. Alternatively, a laser scanning confocal microscope with 32-channel detector and spectral unmixing capability is being used to simultaneously track 4 or more fluorescent proteins and dyes within cells.

Users can also discuss access to specialist equipment for advanced techniques such as fluorescence lifetime imaging microscopy (FLIM) and fluorescence correlation spectroscopy (FCS) or utilise a dedicated workstation for rendering and anlaysis of 3D and 4D image datasets obtained using the facility equipment.

Details

Benefits

Excellent high speed live cell imaging facilities for studying cell, organelle and molecular dynamics.

Access

Please contact the facility manager, Dr Peter Lock, to discuss your imaging requirements and to arrange for training and access to the LIMS Microscopy and Imaging Facility

Access by collaboration, Access by fee for service
Access by users external to managing organisation, Access by users internal to managing organisation

Instruments

Zeiss Cell Observer Spinning disk confocal microscope

spinning disk confocal microscope used for high speed live imaging of cell dynamics.

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Zeiss LSM 510 / Confocor 3 confocal microscope

Laser point scanning confocal microscope used for Live and fixed sample imaging.

Facility: |

Zeiss LSM 780 confocal microscope

confocal laser scanning confocal microscope used for live and fixed multichannel imaging, spectral imaging.

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Location

LIMS Microscopy and Imaging Facility

La Trobe University, Melbourne Campus. LIMS2 Building, Room 104, Science Drive, Bundoora, Victoria, Australia.

View location in Google maps.

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: Northern
Affiliations: La Trobe Institute of Molecular Sciences, La Trobe University

Biological Science categories: Confocal, Fluorescence, Light, Live-cell, Microscopy, Quantitative image analysis