Huygens Software Workshop
See what deconvolution can do for your research! Improve image contrast and resolution while decreasing noise. Correct artifacts such as chromatic shifts, crosstalk, and object movement. This workshop is a great opportunity to get hands-on experience with the Huygens deconvolution software available at The Florey. Suitable for both beginners and experienced microscopists. Important note: bring your own laptop!
In analogy to the people in the cave of the great Greek philosopher Plato who take shadows of men to be real things and echoes to be direct sounds, we tend to believe that what we observe through the microscopic eyepiece is the real truth. But in fact, microscopic images show you no more than what the microscope is willing to tell you about the object being investigated.
Artifacts like blurring, noise, and chromatic aberration all distort your image and affect the ‘true’ object distribution.Consequently, image interpretation and subsequent co-localization analysis and quantitative objects measurements are severely hampered.
Fortunately, the most important distortion factor of a fluorescence microscope – blurring – can be captured as the Point Spread Function (PSF). The PSF is the image of a single point object, and can be regarded as the basic building block from which your acquired image is constructed. The Huygens software uses this PSF to trace back this construction process to the original constituent object, thus restoring it. As a result, image contrast and resolution will be significantly improved, while decreasing noise. Additional artifacts such as chromatic shifts, crosstalk, and object movement can also be measured and corrected by Huygens.
The extensive Huygens workshop on Friday April 11th will start with a short introduction on image formation and deconvolution. We will work with different kind of image data and explore how Huygens deconvolution can be a tool to optimize your microscopic (live cell) imaging experiment, offering you less bleaching issues and faster acquisition time for a wide variety of microscopes, including: widefield, confocal, spinning disc, multi-photon, and superresolution STED systems.
The focus of the workshop will be on deconvolution and the correction of imaging artifacts as a key step in imaging. In addition, we will work with the Huygens advanced 3D-5D visualization, and analysis options .
This workshop is a great opportunity to get some further hands-on experience with the Huygens software and discuss your imaging needs with our imaging specialists. The workshop will be suitable for both beginning and moreexperienced microscopists.
There are only a limited number of places available for this free workshop, so please act fast if you like to attend. You can send an e-mail to firstname.lastname@example.org and email@example.com in order to register for this workshop or ask us for some additional information. Important note: bring your own laptop!