white mouse on blue gloved hand

ES Cell to Mouse & CRISPR Service

Australian Phenomics Network (APN), Monash Univeristy

Contact

Dr. Leanne Hawkey

(613) 9902 4047


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Post author: Leanne Hawkey. Last update: 23/06/2016 at 10:22 am by Karen Clarke.

Special offer

There are no special offers.

Overview

The Australian Phenomics Network (APN) provides Australian researchers with services for the creation, characterisation and cryopreservation of mouse models of human disease.

Established in 2007, the multiple partnering institutions of the APN receive generous funding from the Australian Government’s National Collaborative Research Infrastructure Strategy and Education Investment Fund, with additional funds from the National Health and Medical Research Council, state governments and research institutions, providing Australian researchers access to APN support at substantially subsidised costs.

Three Monash services are proud partners of the APN:

  • The Monash Embryonic Stem (ES) Cell to Mouse service is a core APN facility which provides ready access to the global initiative to systematically inactivate every gene in the mouse genome and generate research models for all 20,000+ genes. Australian researchers choose the genetically modified ES cell line(s) of interest and lodge a request, while the service does the rest – delivering heterozygous genetically modified mice to their facility to advance their research.
  • The newly introduced CRISPR service utilizes the CRISPR/Cas9 genome editing system to modify the rodent genome. Genome modifications can be made by direct injection into fertilized oocytes, resulting in the rapid and efficient generation of founders (F0).Following a thorough evaluation of the technology through a number of pilot projects in several different strains/species, the Monash University node of the Australian Phenomics Network (APN) now offer CRISPR/Cas9 genome editing as an efficient way to generate custom rodent models for clients.
  • The Monash node of the Australian Phenome Bank (APB) is a repository of genetically modified mouse strains. The APB has established an archive consisting of cryopreserved mouse sperm and embryos; maintaining a centralised database detailing mouse strains stored in Australia as live or cryopreserved material. Scientists are encouraged to contribute new strains to this archive and/or search the database for mouse models of interest. Depositing researchers maintain ownership of the strain and can nominate availability to other scientists.

Details

Benefits

Know how:

  • Ordering and Importation of knockout mouse ES cell lines from international knockout mouse consortium
  • Expansion and culturing of mouse ES cell lines
  • Design & production of CRISPR reagents
  • Genotyping via PCR
  • Blastocyst injection of mouse ES cell lines & CRISPR reagents
  • Embryo transfer
  • Breeding chimeric mice to obtain heterozygote offspring
  • Delivery of heterozygote breeding pairs to customer

Access

To access the facility, please contact the Facility Manager, or following the link: http://www.australianphenomics.org.au/services

Access by fee for service
Access by users external to managing organisation, Access by users internal to managing organisation

Instruments

Microinjector

Injection Microscope used for Microinjection of ES cells.

Facility: |

Services

ES Cell injection

Used for Production of mouse models of human disease.

Facility contact: |

Location

Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: South east
Affiliations: Australian Phenomics Network (APN), Monash University
Accreditation: AQIS, OGTR
Biological Science categories: Animal models, Animal services, Bioresources, Cells and tissues, Mouse, Transgenic and Knock-out production

Tags: KO mouse; ES Cell; Cryopreservation