Post author: Belinda Michell. Last update: 02/11/2015 at 2:42 pm by Belinda Michell.
The Zetasizer Nano ZS (Malvern Instruments, UK) instrument uses light scattering techniques to determine the size distribution profile of particles in suspension. There is a well known correlation between the monodispersity of a protein sample and likely crystallisation success. It follows that trialing polydisperse samples in a crystallisation robot would be likely a waste of time and a waste of precious protein. Compared to older DLS technologies, the Zetasizer Nano ZS is very sensitive, capable of handling low microlitre volumes of sample with almost complete sample recovery and is extremely quick, taking only a few minutes per sample. In many respects, DLS data is now as easy to obtain as a conventional UV spectrophotometric reading, and is much more informative about the potential for a sample to yield crystals.
DLS measurements is used by all users of the facility for quality control or prescreening of samples prior to entering crystallisation trials as well as improving any initial crystals obtained. For example, dispersants and additives can be systematically optimised to produce monodisperse solutions of proteins that are much more likely to yield crystals compared to samples containing aggregates. The DLS based approach will further improve the outcomes of the high throughput crystallisation experiments.
- Circular Dichroism.
- X-Ray Crystallography.
- Research collaboration.
- DLS expertise.
Expertise:Availability of technical support:
- Support for instrument use is available.
Note that this material was imported form the Victorian Bioportal and content requires updating.
Zetasizer Nano ZS
The Zetasizer Nano ZS is a high performance two angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentration using dynamic light scattering. used for Dynamic Light Scattering is used to measure particle and molecule size..