Circular Dichroism Spectroscopy

St Vincent

Contact

Ms. Belinda Michell

(613) 9228 2480


You need to login or register to bookmark/favorite this content.

Post author: Belinda Michell. Last update: 02/11/2015 at 2:43 pm by Belinda Michell.

Overview

The CD spectromometer shines polarised light at proteins and allows analysis of protein secondary structure from circular dichroism spectra – for example a-helices and ß-sheets (far UV spectrum) – and aids monitoring of protein folding and conformational changes prior to using more expensive and time consuming methods such as NMR and X-ray crystallography. Secondary structure can be determined by CD spectroscopy in the “far-uv” spectral region. Thermal stability can be assessed using CD by following changes in the spectrum with increasing temperature. CD gives less specific structural information than X-ray crystallography and protein NMR spectroscopy, for example, which both give atomic resolution data. However, CD spectroscopy is a quick method that does not require large amounts of proteins or extensive data processing.

Other infrastructure:

  • Dynamic Light Scattering.
  • X-ray Crystallography.

Access by:

  • Research collaboration.

Details

Benefits

Know how:

  • Circular dichroism.

Expertise:Availability of technical support:

  • Support for instrument use is available.

Note that this material was imported form the Victorian Bioportal and content requires updating.

Access
Instruments

CD Spectrometer

ircular Dichroism spectroscopy measuring in the UV/Vis (163-900nm) spectrum used for A very straightforward, accessible technique to investigate the secondary structure of proteins.

Facility: |

Location

Circular Dichroism Spectroscopy

St Vincent’s Institute of Medical Research, . 9 Princes Street, Fitzroy, Victoria, Australia.

View location in Google maps.

Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: Central
Affiliations: St Vincents Institute of Medical Research

Biological Science categories: NMR and CD spectroscopy, Structural biology