The Werribee Animal Health Facility (WAHF), comprises almost 20 acres of grazing areas, stock yards and animal room accommodation for medium to large animal species requiring QC1 to QC2 levels of microbiological, quarantine approved, containment.
The Transgenic facility has a proven capability in the creation of transgenic rodent models through ES cell transfection and DNA injection techniques including the use of BAC, YAC and viral transgenesis. The facility is able to provide additional advanced reproductive services including Intracytoplasmic Sperm Injection (ICSI mediated transgenesis) and in vitro fertilisation (IVF) procedures.
Custom designed targeting constructs, conditional knock-outs, knock-ins, reporter lines, point mutations, humanised genes, FLP deleter, CRE deleter transgenic constructs, BAC modifications for multiple organisms (mice, rat, human, primate, zebrafish, axolotl) southern screening strategies including cloning of southern probes. CRISPR technology, genotyping strategies, construct design.
DNA constructs are electroporated in our facility into mouse or rat ES cells. After selection, clones are picked and screened by Southern Blot. The correctly targeted clones are then thawed and expanded and only after re-confirmation by Southern and sequencing, they are injected into blastocysts by the Transgenic Services at Monash.
Established through a $2 million grant from the Australian Cancer Research Foundation, the Centre includes a Mediso Nanoscan PET/MRI Imaging Platform, a Mediso Nanoscan SPECT/CT Imaging Platform and an IVIS Spectrum Bioluminescent and Fluorescence Imaging Unit for medical research and preclinical investigations.
Researchers can cryopreserve sperm from their favourite mouse strain for a nominal fee per line^. ^ Conditions apply
Metabolic phenotyping at the BakerIDI
Methodologies used: Microsurgery for induction of heart diseases in mice Echocardiography (non-invasive functional assessment) Micromanometry (hemodynamics) Radiotelemetry for assessment of conscious in vivo cardiovascular parameters Immunohistochemistry and quantitative histology Flow cytometry Routine cellular and biochemical assays
MARP facilities supply and accommodate a wide range of animals for research and teaching projects.
The Australian Phenomics Network (APN) brings together multiple services with world-class infrastructure and expertise for the creation, characterisation and cryopreservation of mouse models of human disease. Generous funding from Federal/ State government, research institutions and NHMRC means the APN can offer researchers access to services at a greatly reduced cost.
AAHL is a world class high-containment facility enabling research and diagnostics on the most dangerous animal and zoonotic pathogens. Extensive laboratories and expertise at physical containment levels 3 and 4 available for collaborative and fee-for-service research.
A state of the art Zebra Fish Research Facility