Targeting and characterizing rodent ES cells
Post author: Arianna Nenci. Last update: 02/11/2015 at 2:57 pm by Arianna Nenci.
The ES cell facility has the capability to produce mouse (Bruce4/C57 and IB10/129) and rat ES cells with specific gene modifications. Working closely with the transgenic suite, the facility prepares ES cells for microinjection and organises screening of the chimeric mice generated, as well as analysis of gem line transmission.
Since 2010 the ES cell facility based in Monash University (Clayton campus) has been providing targeted mouse ES cells to Australian and International research scientists. Our ability and expertise to succeed with even most challenging projects has seen our services widely requested. 41 projects of mouse ES cell targeting have been completed since the opening of the facility with a germ line success rate of 97.5%.
This success allows us to guarantee mouse germ line transmission when projects are fully completed within our facilities including the Gene Recombineering and the Transgenic Services.
The facility also handled 280 different ES cell lines imported from the IKMC, preparing the cells for injections after having validated the targeting.
Dr. Arianna Nenci
Facility Manager at Australian Regenerative Medicine Institute, .
Dr. Arianna Nenci started to work with mouse stem cells at the EMBL (Italy) generating mice models for human disease during her postdoc. At the EMBL she set up the first mouse ES cell facility providing services to internal and external scientists. Arianna has extensive experience targeting and screening mouse and rat ES cells.
P: 03 99029641 | Email |
Dr. Melissa Cooney
Ms. Wendy Chua