ARMI ES Cell Core

Targeting and characterizing rodent ES cells

Contact


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Post author: Arianna Nenci. Last update: 02/11/2015 at 2:57 pm by Arianna Nenci.

Overview

The ES cell facility has the capability to produce mouse (Bruce4/C57 and IB10/129)  and rat ES cells with specific gene modifications. Working closely with the transgenic suite, the facility prepares ES cells for microinjection and organises screening of the chimeric mice generated, as well as analysis of gem line transmission.

Details

Benefits

Since 2010 the ES cell facility based in Monash University (Clayton campus) has been providing targeted mouse ES cells to Australian and International research scientists. Our ability and expertise to succeed with even most challenging projects has seen our services widely requested. 41 projects of mouse ES cell targeting have been completed since the opening of the facility with a germ line success rate of 97.5%.

This success allows us to guarantee mouse germ line transmission when projects are fully completed within our facilities including the Gene Recombineering and the Transgenic Services.

The facility also handled 280 different ES cell lines imported from the IKMC, preparing the cells for injections after having validated the targeting.

Access
People

Dr. Arianna Nenci

Dr. Arianna Nenci

Facility Manager at Australian Regenerative Medicine Institute, .

Dr. Arianna Nenci started to work with mouse stem cells at the EMBL (Italy) generating mice models for human disease during her postdoc. At the EMBL she set up the first mouse ES cell facility providing services to internal and external scientists. Arianna has extensive experience targeting and screening mouse and rat ES cells.

P: 03 99029641 | Email |

Dr. Melissa Cooney

Dr. Melissa Cooney

Facility Staff at Australian Regenerative Medicine Institute, .

P: 03 99029722 | Email |

Ms. Wendy Chua

Ms. Wendy Chua

Facility Staff at Australian Regenerative Medicine Institute, .

P: 03 99029726 | Email |

Instruments
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Services

Electroporation of ES cells

Used for Targeting ES cells to generate Knock out/in mice/rats.

Facility contact: |

Preparation of ES cells for microinjection

Used for Generation of mouse/rat chimeras.

Facility contact: |

Re-confirming of positive clones before injections

Used for Targeting ES cells to generate Knock out/in mice/rats.

Facility contact: |

Screening ES cell clones

Used for Targeting ES cells.

Facility contact: |

Southern blots

Used for Genotyping.

Facility contact: |

Thawing and expanding ES cells

Used for Targeting ES cells to generate Knock out/in mice/rats.

Facility contact: |

Location

ARMI ES Cell Core

Monash University, Clayton Campus. Level2/ Building 75, Wellington Rd, Clayton, Victoria, Australia.

View location in Google maps.

Media

Categories


Type: Core facility in a research organisation
Discipline areas: Biological Science
Precinct: South east
Affiliations: Australian Regenerative Medicine Institute, Monash University

Biological Science categories: Animal models, Bioresources, Cell culture, Cells and tissues, Mouse, Rat

Tags: ES cells, mouse ES cells, rat ES cells, screening ES cells, Southern blot, Targeting ES cells