Used for To screen whether a targeting construct has correctly integrated into the genome.
Post author: Jeanette Rientjes. Last update: 02/02/2016 at 10:03 am by Jeanette Rientjes.
Screening for restriction enzymes that can be used for the digestion of the genomic DNA, cloning of 3′,5′ and if necessary an internal southern probe.
Preferably this is done before the cloning of the targeting construct as restriction enzyme sites can be added to the targeting construct to make the downstream southern screening easier.
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