Southern Screening Strategies

Used for To screen whether a targeting construct has correctly integrated into the genome.

Actions

You need to login or register to bookmark/favorite this content.

Post author: Jeanette Rientjes. Last update: 02/02/2016 at 10:03 am by Jeanette Rientjes.

Overview

Ms. Jeanette Rientjes

Phone: 03 9902 9730

Details

Service overview

Screening for restriction enzymes that can be used for the digestion of the genomic DNA, cloning of 3′,5′ and if necessary an internal southern probe.
Preferably this is done before the cloning of the targeting construct as restriction enzyme sites can be added to the targeting construct to make the downstream southern screening easier.

Additional info

Tags

Capacity: We encourage greater utilisation
Operation: Facility provides the service for you
Access: Access by fee for service