small scale optimization E.coli

Used for Identification of expression conditions for recombinant proteins in E.coli strains

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Post author: Noelene Quinsey. Last update: 01/07/2014 at 5:31 pm by Noelene Quinsey.

Overview

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Details

Service overview

Expression optimization of recombinant proteins in a variety of E.coli strains using different factors that effect recombinant protein expression within E.coli such as temperature, incubation length and media.

Additional info

Majority of proteins tested in this service have some sort of affinity tagged attached to them eg His, MBP, GST or strep tag. In some cases, optimization can be carried out using a construct that doesn’t carry an affinity tag but the information generated is limited.

Tags

Capacity: We encourage greater utilisation
Operation: Facility provides the service for you
Access: Access by collaboration, Access by fee for service

Tags: E.coli, small scale optimization of recombinant proteins