Used for Identification of expression conditions for recombinant proteins in E.coli strains
Post author: Noelene Quinsey. Last update: 01/07/2014 at 5:31 pm by Noelene Quinsey.
Expression optimization of recombinant proteins in a variety of E.coli strains using different factors that effect recombinant protein expression within E.coli such as temperature, incubation length and media.
Majority of proteins tested in this service have some sort of affinity tagged attached to them eg His, MBP, GST or strep tag. In some cases, optimization can be carried out using a construct that doesn’t carry an affinity tag but the information generated is limited.