Screening ES cell clones

Used for Targeting ES cells

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Post author: Arianna Nenci. Last update: 27/06/2014 at 4:22 pm by Arianna Nenci.

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Once mouse or rat ES cells are elctroporated with specific DNA constructs, the picked clones are screened to identify the correctly targeted ones. The screening is performed by Southern blot using probes hybridizing externally the construct on both 5′ and 3′ arms oh homology. This is the only way to distinguish a homologous recombinants from a random integrants. If the DNA constructs is cloned at the Gene Recombineering facility at Monash, the southern strategy is validated in advance on genomic wild type DNA.

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