Re-confirming of positive clones before injections

Used for Targeting ES cells to generate Knock out/in mice/rats

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Post author: Arianna Nenci. Last update: 30/06/2014 at 12:28 pm by Arianna Nenci.

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Service overview

Before proceeding with the injection into blastocysts, the chosen clones are re-confirmed by Southern blot (5′, 3′ and internal probes) and by sequencing important sites such as mutations, LoxPs and FRTs.

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Capacity: We encourage greater utilisation
Operation: Facility provides the service for you
Access: Access by fee for service