Used for Targeting ES cells to generate Knock out/in mice/rats
Post author: Arianna Nenci. Last update: 30/06/2014 at 12:28 pm by Arianna Nenci.
Before proceeding with the injection into blastocysts, the chosen clones are re-confirmed by Southern blot (5′, 3′ and internal probes) and by sequencing important sites such as mutations, LoxPs and FRTs.
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