Mouse and Rat ES cell injection into blastocysts

Used for Generation of knock out/in mice and rats

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Post author: Jose Gonzalez. Last update: 27/06/2014 at 2:25 pm by Jose Gonzalez.

Overview

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Details

Service overview

The Transgenic facility has a proven capability in the creation of knock out/in rodent models through ES cell injections for the generation of chimeric animals. Pups can be bred to the heterozygous stage when requested. If the projects are completely developed in our facilities, from construct to the animal, we guarantee germ line transmission.

Additional info

When the projects are completely developed in our facilities, from construct to the animal, we guarantee germ line transmission. Working in close collaboration with the ES cell core, the facility can inject outsourced cells, helping with the importation if coming from oversees (EUCOMM, KOMP, Sanger). Once the heterozygous are generated, the pups are genotyped by PCR and confirmed by Southern blot.

Tags

Capacity: We encourage greater utilisation
Operation: Facility provides the service for you
Access: Access by fee for service

Tags: conditional, knock in, knock out