Used for Generation of transgenic mice and rats
Post author: Jose Gonzalez. Last update: 27/06/2014 at 2:24 pm by Jose Gonzalez.
Genetically modified rodents can be generated using the recently discovered CRISPR/Cas Nuclease RNA-guided genome editing technology.
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. The discovery of the type II prokaryotic CRISPR “immune system” has allowed for the development for an RNA-guided genome editing tool that is simple, easy and quick to implement.
Recent studies have shown the CRISPR/Cas system used for gene editing (silencing, enhancing or changing specific genes) in animal models including mice and primates. One-step generation of genome targeting has been achieved by co-microinjection of one cell stage embryos with Cas9 mRNA and small/single guide (sg) RNA specifically designed for the gene of interest, allowing an organism’s genome to be modified at any desired location.
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