Confocal laser scanning microscope used for optical sectioning, fixed tissue
Post author: Verena Wimmer. Last update: 20/05/2015 at 3:29 pm by Verena Wimmer.
Manufacturer: Zeiss, LSM 780. Installed 2013.
Confocal laser scanning microscope with 405, 458, 488, 514, 561, 594 and 633 nm laser lines, 32-channel high-sensitivity GaAsP detector and two side PMTs, photon counting mode, motorised xy-stage, 20x dipping lens for imaging in cleared brains.
Benefits of the Zeiss LSM 780
Simultaneous imaging of up to ten channels, even dim samples will come to light with highly sensitive GaAsP detector array, mosaic scanning with convex hull function to decrease imaging time, long working distance 20x NA1.0 dipping lens to make full use of recent technical advances in optical clearing of brain samples (can be used for SCALEVIEW as well as CLARITY cleared samples).
Technical features and specifications
Zeiss Axio Observer Upright Microscope
405, 458, 488, 514, 561, 594, 633 (Class 3B lasers)
PL-APO 10x/0.45 (DIC); PL-APO 20x/0.8 (DIC); PL-APO 40x/1.4 (DIC); PL-APO 63x/1.4 (DIC). PL-Neofluar 63x/1.3 glyc (DIC) ; WPlanAPO 20x/1.0 DIC(UV) VIS-IR (1.8mm working distance)
2 flanking fluorescence PMTs, 32 channel GaAsP array + 1 transmitted
Zeiss ZEN software including for normal acquisition, including 3D, co-localisation, multidimensional data/series acquisition (avi movies). Conventional multi-channel imaging, series acquisition, 3D-6D, reconstruction