A laser capture system used for to “dissect” cells from tissue sections
Post author: Paul McMillan. Last update: 02/09/2015 at 9:34 am by Paul McMillan.
Anatomy & Neuroscience, Parkville.
Manufacturer: Zeiss, PALM
The laser capture microscope uses a UV laser to first microdissect tissue sections and then catapult the dissected piece of tissue into an Eppendorf tube. The UV wavelength of the laser is too long to damage polynucleotides. The same system is also fitted with an infra-red laser that acts as optical tweezers for the manipulationof single cells and smallparticles. The system is fitted to a Zeiss inverted fluorescence microscope
Benefits of the PALM laser microdissection
Perform genomic, qPCR or proteomics analysis on specific regions of tissue.
Technical features and specifications
Inverted Zeiss fluorescence microscope
Objective: 10x Air (0.3NA), 20x Air (0.5NA), 40x Air (0.6NA), 63x Oil (1.25NA), 100x Oil (1.3NA)
Excitation/lasers: Mercury bulb, 355nm (Laser microdissection) and 1064nm (Laser capture)
Emission filters: DAPI, FITC, Texas Red
Detectors: Zeiss Mr Camera