Mass spectrometer used for proteomics
Post author: Nicholas Williamson. Last update: 31/08/2015 at 3:45 pm by Nicholas Williamson.
Manufacturer: Thermo Fisher, OrbiTRAP ELITE ETD. Installed 2013.
This instrument and the attached Dionex U3000 nanoLC is dedicated to protein and peptide analysis. Typical experiments include gel spot analysis, SILAC, iTRAQ, label free quantitiation, ETD fragmentation of phosphopeptides, shot gun analysis of protein mixtures.
Funding sources: ARC LIEF, Institutional
Benefits of the OrbiTRAP ELITE ETD
This instrument offers very high mass resolution and the ability to fragment peptides and proteins using ether CID, HCD or ETD methods. It is setup with a nano flow HPLC system to allow the best possible sensitivity for low sample amounts
Technical features and specifications
ETD, CID or HCD fragmentation