SIM super-resolution microscope used for structured illumination microscopy, live cell imaging, high speed deconvolution microscopy
Post author: Lauren Hyde. Last update: 24/04/2015 at 11:41 am by Gerard Gibbs.
Manufacturer: Applied Precision, V4 Blaze. Installed 2011.
Conventional optical microscopes have a fundamental resolution limit of approximately 250nm, depending on wavelength. The OMX V4 Blaze, by applying a structured illumination approach, can pass this limit and obtain a maximum resolution of 100nm in x/y. It is fitted with multiple lasers (405, 488, 568 and 642nm) and three cameras for maximum speed.
Funding sources: Other (Melbourne Materials Institute and the School of Engineering)
Benefits of the OMX V4 Blaze
The super-resolution capabilities, down to 100 nm in x/y and 250nm in z, allow samples to be imaged in unparalleled detail. The microscope is especially well suited to studying the internal structure of cells at this scale, such as probing the internalisation behaviour of nano particles, protein-protein interaction or imaging cell-to-cell signalling.
In conventional, non super-resolution mode, the OMX V4 Blaze can also image at speeds in excess of 100 frames per second across three channels simultaneously.
Technical features and specifications
Super-resolution or high speed conventional microscopy:
Available laser lines for super-resolution:
Fitted with incubation chamber for live cell imaging.
We encourage greater utilisation
Operation: User operated training provided, User or facility staff operate, User or facility staff operate pricing may vary
Access: Access pricing may vary, External researcher use availableCapacity: