OMX V4 Blaze

Instrument: OMX V4 Blaze

SIM super-resolution microscope used for structured illumination microscopy, live cell imaging, high speed deconvolution microscopy

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Post author: Lauren Hyde. Last update: 24/04/2015 at 11:41 am by Gerard Gibbs.

Overview

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Details

Overview

Manufacturer: Applied Precision, V4 Blaze. Installed 2011.

Conventional optical microscopes have a fundamental resolution limit of approximately 250nm, depending on wavelength. The OMX V4 Blaze, by applying a structured illumination approach, can pass this limit and obtain a maximum resolution of 100nm in x/y. It is fitted with multiple lasers (405, 488, 568 and 642nm) and three cameras for maximum speed.

Funding sources: Other (Melbourne Materials Institute and the School of Engineering)

Benefits of the OMX V4 Blaze

The super-resolution capabilities, down to 100 nm in x/y and 250nm in z, allow samples to be imaged in unparalleled detail. The microscope is especially well suited to studying the internal structure of cells at this scale, such as probing the internalisation behaviour of nano particles, protein-protein interaction or imaging cell-to-cell signalling.

In conventional, non super-resolution mode, the OMX V4 Blaze can also image at speeds in excess of 100 frames per second across three channels simultaneously.

Technical features and specifications

Super-resolution or high speed conventional microscopy:

  • Super-resolution: x/y resolution 100 nm, z resolution 250nm
  • Conventional imaging: three cameras for fast simultaneous imaging greater than 100 fps in three colours
  • Available laser lines for super-resolution:

  • 405 nm
  • 488 nm
  • 568 nm
  • 642 nm
  • Fitted with incubation chamber for live cell imaging.

    Tags

    Capacity: We encourage greater utilisation
    Operation: User operated training provided, User or facility staff operate, User or facility staff operate pricing may vary
    Access: Access pricing may vary, External researcher use available