Olympus FV1000

Instrument: Olympus FV1000

Confocal laser scanning microscope used for optical sectioning, live cell imaging

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Post author: Verena Wimmer. Last update: 01/04/2014 at 5:01 pm by Verena Wimmer.

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Overview

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Details

Overview

Manufacturer: Olympus, FV1000. Installed 2007.

Filter-based multi-channel acquisition. SIMultaneous Twin Scanner for 3D-6D, live cell imaging, time lapse, ratio-metric, FRAP, and FRET

Benefits of the Olympus FV1000

Set up for high speed imaging of fast cellular events (for example, calcium imaging) as well as intermediate-term live-cell imaging in a temperature and CO2 controlled chamber. Study molecular dynamics and interactions using FRAP and FRET.

Technical features and specifications

Olympus IX81 Inverted Microscope
405,440, 473, 559,635 nm diode Class 3B lasers
UPLSAPO10x (DIC) UPLSAPO20x (DIC) UPLNFLN40X (DIC) UPLSAOP60X (DIC) UPLSAPO100x PLAPO60X (DIC)
3 Fluorescence PMT+ 1 transmitted detector (for BF, DIC (10x, 20x, 40x, 63x)
Olympus Confocal Software for normal acquisition, including 3D, co-localisation, multidimensional data/series acquisition (avi movies). SIMultaneous Twin Scanner Conventional multi-channel imaging, series acquisition, 3D-6D, live cell imaging (heated and temperature controlled on stage incubator), time lapse, ratio-metric, FRAP, FRET, reflection, reconstruction

Tags

Capacity: We encourage greater utilisation
Operation: User operated training provided
Access: Access pricing may vary, External researcher use available