Confocal laser scanning microscope used for optical sectioning, live cell imaging
Post author: Verena Wimmer. Last update: 01/04/2014 at 5:01 pm by Verena Wimmer.
Manufacturer: Olympus, FV1000. Installed 2007.
Filter-based multi-channel acquisition. SIMultaneous Twin Scanner for 3D-6D, live cell imaging, time lapse, ratio-metric, FRAP, and FRET
Benefits of the Olympus FV1000
Set up for high speed imaging of fast cellular events (for example, calcium imaging) as well as intermediate-term live-cell imaging in a temperature and CO2 controlled chamber. Study molecular dynamics and interactions using FRAP and FRET.
Technical features and specifications
Olympus IX81 Inverted Microscope
405,440, 473, 559,635 nm diode Class 3B lasers
UPLSAPO10x (DIC) UPLSAPO20x (DIC) UPLNFLN40X (DIC) UPLSAOP60X (DIC) UPLSAPO100x PLAPO60X (DIC)
3 Fluorescence PMT+ 1 transmitted detector (for BF, DIC (10x, 20x, 40x, 63x)
Olympus Confocal Software for normal acquisition, including 3D, co-localisation, multidimensional data/series acquisition (avi movies). SIMultaneous Twin Scanner Conventional multi-channel imaging, series acquisition, 3D-6D, live cell imaging (heated and temperature controlled on stage incubator), time lapse, ratio-metric, FRAP, FRET, reflection, reconstruction