Confocal laser scanning microscope used for optical sectioning, fixed tissue
Post author: Verena Wimmer. Last update: 20/05/2015 at 3:28 pm by Verena Wimmer.
Manufacturer: Leica, SP8. Installed 2013.
Confocal laser scanning microscope with 405, 458, 476, 488, 496, 514, 561 and 633 nm laser lines and AOBS gated detection, five channels with two high sensitivity HyD detectors and three PMTs, motorized xy-stage.
Benefits of the Leica TCS SP8
Fast imaging of up to five channels simultaneously, high flexibility with acousto-optic beam splitter (AOBS), excellent imaging of dim staining using sensitive HyD detectors with outstanding quantum efficiency.
Technical features and specifications
Leica DMI6000 inverted microscope
405,458,476,488,496,514,561,633 (Class 3B lasers)
HC PL APO 10x/0.4 (DIC); HC PL FL 20x/0.5 (DIC); HC PL APO 40x/1.3 (DIC); HCX PL FL 40x/0.6 long working distance (LWD); HC PL APO 63x/1.4 (DIC); HC PL APO 63x/1.30 glyc
3 PMTs, 2 HyD GaAsP detectors+ 1 transmitted detector
LAS Software including for normal acquisition, including 3D, co-localisation, multidimensional data/series acquisition (avi movies).
Conventional multi-channel imaging, series acquisition, 3D-6D, reconstruction