In vivo and in vitro 3D Optical molecular imaging system used for Bioluminescence & Fluorescence, Transmission & Reflectance Imaging
Post author: Kerryn Westcott. Last update: 18/06/2015 at 12:10 pm by Kerryn Westcott.
Manufacturer: Caliper Life Sciences, IVIS Spectrum. Installed 2011.
The IVIS® Spectrum is a versatile and advanced in vivo imaging system which uses a novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near-infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using a Digital Mouse Atlas or registered with the IVIS multimodality module to other tomographic technologies such as MR, CT or PET.
For advanced fluorescence pre-clinical imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.
Funding sources: ACRF
Benefits of the IVIS Spectrum
Full details can be obtained from the equipment manager.
Technical features and specifications
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