3D Structured Illumination Microscope

Super resolution microscope used for Fluorescence imaging at 125nm resolution


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Post author: Paul McMillan. Last update: 28/02/2014 at 2:07 pm by Paul McMillan.






Manufacturer: Applied Precision, OMX Blaze

Access to the OMX system is on a collaborative basis with one of the super users. Please click the link for more info to see the list of superusers.

Funding sources: ARC LEIF

Benefits of the 3D Structured Illumination Microscope

3D Structured Illumination (3D-SIM) is a super-resolution optical microscopy method that produces an 8-fold increase in resolution in multiple channels using standard fluorophores

Technical features and specifications

Microscope stand – Inverted system with temperature & gas control
Objective – 60x oil immersion
Excitation lines – 405, 445, 488, 514, 568, 642 nm
Emission filters (Band Pass) – 435, 477, 528, 550, 608, 683 nm
Detectors – Four sCMOS cameras can image 4 colours simultaneously

Price list

Pricing category Price
University of Melbourne $70/hr
WEHI $70/hr
Monash University $70/hr
La Trobe University $70/hr
Other Universities/Institutes $100/hr
Industry $200/hr


Capacity: We encourage greater utilisation
Operation: Facility staff operate this instrument
Access: Access pricing may vary, External researcher use available