Super resolution microscope used for Fluorescence imaging at 125nm resolution
Post author: Paul McMillan. Last update: 28/02/2014 at 2:07 pm by Paul McMillan.
Bio21 Institute, Parkville.
Manufacturer: Applied Precision, OMX Blaze
Access to the OMX system is on a collaborative basis with one of the super users. Please click the link for more info to see the list of superusers.
Funding sources: ARC LEIF
Benefits of the 3D Structured Illumination Microscope
3D Structured Illumination (3D-SIM) is a super-resolution optical microscopy method that produces an 8-fold increase in resolution in multiple channels using standard fluorophores
Technical features and specifications
Microscope stand – Inverted system with temperature & gas control
Objective – 60x oil immersion
Excitation lines – 405, 445, 488, 514, 568, 642 nm
Emission filters (Band Pass) – 435, 477, 528, 550, 608, 683 nm
Detectors – Four sCMOS cameras can image 4 colours simultaneously
|University of Melbourne||$70/hr|
|La Trobe University||$70/hr|